YPAD Agar Size:1000g

YPAD Agar Size:1000gFormula g l Yeast extract 10 Peptone 20 Glucose 20 Adenine Sulfate 0. 04 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water Store dry at room temperature.

Suspend 12

Biotin is omitted from this formulation of Yeast Nitrogen base w/o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin

2 at 25°C Suspend 50 gram powdered medium in 1 litre distilled water Store dry at room temperature

0002% Sulfate (SO4) <0

Usage above a 20 mM concentration in mammalian cell culture work is not recommended

02 Cyanocobalamin 0

2 at 25°C Suspend 16 gram powdered medium in 1 litre distilled water

5 Ascorbic Acid 0

Peptides and amino acids are abundantly present in Tryptone

5M water ≤0

Allow bottle to stand to room temperature before opening

20mM Glacial Acetic Acid and 1mM Na2EDTA